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ＭＩＰ－１α是一种对多种免疫效应细胞包括Ｔ细胞、Ｂ细胞、单核细胞及中性粒细胞等具有显著趋化作用的趋化因子。通过重组腺病毒载体将ＭＩＰ－１α基因导入Ｂ１６黑色素瘤细胞后，对其体内外生物学特性进行了初步的研究。结果表明，ＭＩＰ－１α基因转染后Ｂ１６黑色素瘤细胞体外生长特性无明显变化，其培养上清中ＭＩＰ－１α的分泌水平可达３６８±２４ｎｇ／ｍｌ／１０６／２４ｈ，上清对ＮＫ细胞、ＣＤ４＋Ｔ细胞、ＣＤ８＋Ｔ细胞及巨噬细胞均具有极强的趋化作用。ＭＩＰ－１α基因转染的肿瘤细胞接种至小鼠皮下后，肿瘤生长明显减慢，肿瘤局部有大量的炎性细胞浸润；然而荷瘤小鼠的存活期未见明显增强，其ＮＫ活性及ＣＴＬ活性也未见增强，推测其原因与体内ＭＩＰ－１α所致的非特异性炎症反应过强有关。
MIP-1α is a chemotactic factor that has a significant chemotactic effect on various immune effector cells including T cells, B cells, monocytes and neutrophils. After the MIP-1α gene was introduced into B16 melanoma cells by recombinant adenovirus vector, the in vitro and in vivo biological characteristics of MIP-1α gene were studied. The results showed that the MIP-1α gene transfection in B16 melanoma cells in vitro growth characteristics did not change significantly in the culture supernatant MIP-1α secretion of up to 368 ± 24ng / ml / 106 / 24h, the supernatant of NK cells, CD4 + T cells, CD8 + T cells and macrophages are extremely chemotactic. MIP-1α gene transfected tumor cells inoculated into mice subcutaneously, the tumor growth was significantly slowed down, the tumor has a large number of local inflammatory cell infiltration; However, the survival of tumor-bearing mice showed no significant increase in its NK activity and CTL Activity has not been enhanced, speculated that the reason and the body caused by MIP-1α non-specific inflammatory response is too strong.