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新鲜采取的尼罗罗非鱼精液在加入棉酚的精子保存液中在２８℃下培养。高达１０ｍＭ的乙酸棉酚也不影响精子在一小时内的存活率。罗非鱼精子的存活率在培养三小时后存活率显著下降，而低浓度棉酚（２５－１００μＭ）显著提高罗非鱼精子的存活中。本文对上述实验结果进行了讨论。
Freshly taken nile tilapia semen is incubated in gossypol-added sperm pool at 28 ° C. Acetate gossamer up to 10 mM also did not affect sperm survival in one hour. Survival rates of tilapia sperm decreased significantly after three hours of culture, whereas low concentrations of gossypol (25-100 μM) significantly increased the survival of tilapia sperm. This article discusses the above experimental results.