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【目的】研究广州市公共场所中央空调冷却塔水中军团菌的基因特征和优势型别。【方法】采用军团菌巨噬细胞感染力增强因子(Macrophage infectivity potentiator,mip)基因分型方法。提取广州市2008-2010年分离的140株(119株嗜肺,21株非嗜肺)军团菌基因组DNA,针对mip基因进行PCR扩增并测序,将核苷酸序列上传至欧洲军团菌感染工作组(EWGLI)数据库进行比对,得到mip型别,并构建系统发育进化树。【结果】140株军团菌均可扩增出700 bp左右的目的条带。119株嗜肺军团菌分为10个mip型别,L.pneumophila-phil-1为优势型别,占52.9%(63/119);21株非嗜肺军团菌分为6个mip型别,L.feeleii-D3131为优势型别,占47.6%(10/21)。【结论】广州市公共场所中央空调冷却塔水中军团菌具有多样性,mip分型技术可用于军团菌的快速基因分型。
【Objective】 The objective of this study was to investigate the genetic characteristics and dominant genotypes of Legionella in water of central air conditioning cooling towers in public places in Guangzhou. 【Method】 Legionella macrophage infectivity potentiator (mip) genotyping method was used. Genomic DNA of 140 Legionella strains (119 strains of pneumoconiosis and 21 strains of non-pneumonic bacteria) isolated in Guangzhou from 2008 to 2010 was extracted. The mip gene was amplified by PCR and sequenced, and the nucleotide sequence was uploaded to Legionella pneumophila (EWGLI) database to obtain the mip type, and to construct phylogenetic tree. 【Result】 The results showed that all the 140 Legionella strains could amplify the target band of about 700 bp. 119 strains of Legionella pneumophila were divided into 10 mip types, L. pneumophila-phil-1 was the dominant type, accounting for 52.9% (63/119); 21 non-Pneumophila strains were divided into 6 mip types, L.feeleii-D3131 was the dominant type, accounting for 47.6% (10/21). 【Conclusion】 Legionella in water of central air conditioning cooling towers in public places in Guangzhou are diversified. The mip typing technology can be used for rapid genotyping of Legionella.