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目的 检验神经元cdc 2类激酶 (Cdk5/p35nck5a)在神经元成熟和萌芽中扮演着重要作用的假说。方法 采用Westernblot分析 ,免疫组织化学染色和免疫沉降Cdk5活性分析的方法研究了在癫痫各阶段大鼠海马区神经元cdc 2类激酶的活性变化、Cdk5和p35nck5a的表达水平及细胞内的分布。结果 在癫痫大鼠脑中的活性比正常大鼠脑中的活性呈有意义的增高 ,在所有癫痫各阶段的神经元cdc 2类激酶活性中 ,第三阶段的活性是最高的。该激酶活性的变化与p35nck5a表达的变化是一致的。Cdk5的表达量在癫痫各阶段中是恒定不变的 ,但是 ,它在细胞内分布出现了引人注目的变化 ,当Cdk5活性高时Cdk5的分布从轴索转移到细胞质 ,p35nck5a的分布一直在细胞质内。结论 神经元cdc 2类激酶在轴索突触再组成上扮演着重要的作用 ,而且 ,Cdk5的分布从轴索转移到细胞质的过程可能是控制其活性的一个新调控机理
Objective To test the hypothesis that the neuronal cdc type 2 kinase (Cdk5 / p35nck5a) plays an important role in the maturation and germination of neurons. Methods The changes of cdc 2-kinase activity, the expression of Cdk5 and p35nck5a and the intracellular distribution of the neurons in the hippocampus of rats at various stages of epilepsy were studied by Western blot analysis, immunohistochemical staining and immunoprecipitation of Cdk5 activity assay. As a result, the activity in the brain of epilepsy rat was significantly increased compared with that in normal rat brain, and the activity of the third phase was the highest among the neuronal cdc class 2 kinase activities in all stages of epilepsy. Changes in this kinase activity are consistent with changes in p35nck5a expression. The expression level of Cdk5 is constant in all stages of epilepsy. However, its distribution in the intracellular has a dramatic change. When the Cdk5 activity is high, the distribution of Cdk5 is transferred from the axon to the cytoplasm. The distribution of p35nck5a Cytoplasm. Conclusions Neuronal cdc 2-type kinases play an important role in axonal synaptic reorganization. Furthermore, the process of Cdk5 distribution from axonal to cytoplasm may be a new regulatory mechanism that controls its activity