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用扫描电镜观察了刀豆素A诱导小鼠胸腺细胞成帽的形貌特征及动力学变化。经F—ConA孵育后的胸腺细胞有三种类型:微聚集型,可能为配体(ConA)与受体结合后在细胞上的早期表现;块状聚集型,可能为过渡型;帽型,有乳头状、锥状和盔状三种。F—ConA与胸腺细胞孵育10mim成帽率达到高峰(30.3％),但2mim亦有16.9％的细胞成帽,说明成帽速度是很快的。经秋水仙硷处理的胸腺细胞,成帽率明显增高,且在2mim达到高峰(50％),说明微管的破坏既提高了成帽率又加快了成帽速度。秋水仙硷处理的胸腺细胞与F—Con孵育2min后,随时间的推移,成帽率呈递减趋势,其原因有待于进一步探讨。
Scanning electron microscopy was used to observe the morphology and kinetics of capping of mouse thymocytes induced by Concanavalin A. There are three types of thymocytes incubated with F-ConA: microaggregated, which may be the early expression of the ligand (ConA) after binding to the receptor; blocky aggregation, possibly transitional; cap, with There are three kinds of nipples, cones and helmets. F-ConA incubated with thymocytes incubated 10mim cap rate reached a peak (30.3%), but 2mim also 16.9% of cells capped, indicating that the cap speed is very fast. The percentage of capped thymocytes treated with colchicine was significantly higher and peaked at 2mim (50%), indicating that the destruction of microtubules not only increased the cap rate but also accelerated the rate of cap formation. When the thymocytes treated with colchicine were incubated with F-Con for 2 min, the cap rate showed a decreasing trend with time, and the reasons for this need to be further explored.