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【目的】分离与鉴定人肠道乳酸利用菌,研究其乳酸利用的代谢特性。【方法】利用Hungate滚管技术从人粪便中分离厌氧细菌,通过形态、生化和16S r RNA基因鉴定;通过体外发酵技术研究乳酸代谢,并且与乳酸产生菌共培养,研究二者之间交互饲喂。【结果】验证了乳酸可作为代谢中间产物被人肠道混合菌群利用;分离到一株乳酸利用菌,在24 h内消耗乳酸超过50 mmol/L,经鉴定为韦荣氏球菌,并将其形成乙酸和丙酸。当和肠球菌共培养时,可以有效地减缓乳酸的积累。【结论】本株乳酸利用菌可以作为潜在的益生菌,和乳酸菌一起调节人肠道的乳酸动态平衡。
【Objective】 Isolation and identification of human intestinal lactic acid bacteria, to study the metabolic characteristics of lactic acid utilization. 【Method】 Anaerobic bacteria were isolated from human excrement by the Hungate roller technique and identified by morphological, biochemical and 16S rRNA genetics. Lactate metabolism was studied by in vitro fermentation and co-cultured with lactic acid producing bacteria to study the interaction between the two Feeding. 【Result】 The results showed that lactic acid could be used as a metabolic intermediate for human intestinal mixed bacteria. A lactic acid-utilizing bacterium was isolated, which consumed more than 50 mmol / L lactic acid within 24 h and was identified as Veillonella. It forms acetic acid and propionic acid. When co-cultured with enterococci, can effectively slow down the accumulation of lactic acid. 【Conclusion】 This strain of lactic acid-utilizing bacterium can be used as potential probiotics to regulate lactic acid balance in human intestine with lactic acid bacteria.