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目的 探索放射联合肿瘤引流区淋巴结淋巴细胞 (tumordraininglymphnodelymphocyte,DNL)对肿瘤细胞的作用。方法 取首次治疗病理证实的口腔癌患者颈清扫淋巴结 ,经常规消化、分离 ,加白细胞介素 2 (interleukin 2 ,IL 2 )培养 ,获取激活的淋巴细胞作为效应细胞 ,以效靶比 (E∶T) 2 5∶1进行集落形成试验 ,观察对人涎腺腺样囊性癌肺高转移细胞株 (adenoidcysticcarcinoma ,ACC M)的抗瘤效应 ,并分别以不同剂量60 钴γ射线对ACC M照射后 ,随即加入DNL细胞 ,观察放射结合DNL对ACC M的抗瘤效应。结果 ACC M为放射中度敏感细胞。根据线性———二次数学模型 (LQ模型 ) ,其α、β值分别为 0 342 0 ,0 0 783。DNL对ACC M的抑瘤率为 49 0 6 %。放射联合DNL作用时 ,α值提高到 0 76 88;Dq、S2 分别从 1 5 90 1、0 44 81降至 0 5 995、0 113 5 ,这 3个参数与放射组相比 ,差异均有显著性 (P <0 0 5 )。在高剂量区 ,与放射组比 ,放射联合淋巴细胞组的D0 0 1和 6Gy、8Gy剂量点的细胞存活率均明显下降 ,P <0 0 1。结论 体外来源于不同病理类型口腔癌的IL 2激活的DNL对ACC M有明显的抗瘤效应 ,无论在低剂量还是高剂量区与放射结合都有显著的协同抗瘤效应
Objective To explore the effect of radiation combined with tumordraining lyophilized lymphode lymphocyte (DNL) on tumor cells. Methods The cervical lymph nodes were initially treated with pathologically confirmed oral cancer. The cells were routinely digested, separated, cultured with interleukin 2 (IL 2), and activated lymphocytes were obtained as effector cells. The effective target ratio (E: T) 25: 1. The antitumor effects on human salivary adenoid cystic carcinoma cells (ACC M) were observed. The effects of 60 Co γ -ray on ACC M After that, DNL cells were added to observe the anti-tumor effect of radioactive DNL on ACC M cells. Results ACC M was moderately sensitive to radiation. According to the linear-quadratic mathematical model (LQ model), the α and β values are 0 342 0 and 0 0 783, respectively. The inhibition rate of DNL to ACC M was 49 0 6%. In the combination of radiation and DNL, the α value increased to 0 76 88; Dq and S2 decreased from 1 5 90 1,044 81 to 0 5 995 0 and 113 5, respectively Significance (P <0 05). In the high dose area, the cell viability at D0 0 1, 6Gy, and 8Gy dose-dependently decreased significantly compared with that of the radiotherapy group (P <0.01). Conclusion DNL-activated DNL derived from different pathological types of oral cancer in vitro has obvious anti-tumor effect on ACC M, and has significant synergistic anti-tumor effect both in low-dose and high-dose areas