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为了验证水稻(Oryza sativa L.)细胞质型APXs与细胞耐盐性的关系,实验分别将OsAPXa和OsAPXb(基因登录号:D45423、AB053297)转化到烟草(Nictiana tabacum,N.plum)植株中。Southern结果表明,二基因分别整合到烟草的基因组;Northern分析表明,外源基因在转基因烟草中得到高效表达;在碳酸盐逆境下,T2代转基因植株与野生型对照相比,其APX活性呈现显著的提高,T2代品系的H2O2含量和叶片受害程度显著低于野生型;T2代品系分别在含有10mmol·L-1NaHCO3、5mmol·L-1Na2CO3的MS培养基上生长,根的生长受到抑制,叶片产生黄化;野生型烟草则难以存活。水稻细胞质型OsAPXs基因的过量表达提高了转基因烟草的耐盐性,揭示出Os-APXa和OsAPXb在碳酸盐逆境应答过程中发挥着重要的作用。
In order to verify the relationship between cytoplasmic APXs and salt tolerance in rice (Oryza sativa L.), OsAPXa and OsAPXb (gene accession number: D45423, AB053297) were transformed into Nicotiana tabacum (N. Southern results showed that the two genes were integrated into the genome of tobacco respectively. Northern analysis showed that the exogenous genes were highly expressed in transgenic tobacco. Under carbonate stress, compared with the wild-type controls, the T2 generation transgenic plants exhibited APX activity H2O2 content and leaf damage degree of T2 generation were significantly lower than that of wild type. T2 generation grew on MS medium containing 10 mmol·L-1NaHCO3 and 5 mmol·L-1Na2CO3 respectively, the growth of root was inhibited, Leaves produce yellowing; wild-type tobacco is difficult to survive. Overexpression of rice cytoplasmic OsAPXs increased the salt tolerance of transgenic tobacco and revealed that Os-APXa and OsAPXb play an important role in the response of carbonate stress.