论文部分内容阅读
目的:研究双歧杆菌对新生鼠坏死性小肠结肠炎模型中肠道组织Toll样受体(TLR)2、4、9表达的影响。方法:出生24 h内Sprague-Dewley新生鼠随机分为对照组、实验组、治疗组,每组12只。对照组由母鼠喂养,实验组采取人工喂养+缺氧冷刺激进行造模,治疗组采用人工喂养+缺氧冷刺激并添加喂养双歧杆菌处理(2次/日,1×108cfu/次)。每日定时称体重做记录,在实验第3天处死新生鼠。用HE染色病理切片观察新生鼠肠道病理变化,RT-PCR方法检测肠道黏膜TLR2、TLR4、TLR9的mRNA表达量。结果:3组小鼠体重比较均具有统计学意义,对照组体重增长高于实验组及治疗组,治疗组体重增长高于实验组(P<0.05);实验组病理评分高于对照组及治疗组,治疗组高于对照组(P<0.05);TLR4 mRNA相对表达量实验组高于对照组及治疗组(P<0.05),治疗组的TLR2 mRNA、TLR9 mRNA相对表达量均高于实验组,TLR4 mRNA相对表达量低于实验组,这两组的3种受体mRNA相对表达量比较均具有统计学意义(P<0.05)。结论:双歧杆菌可减轻新生鼠坏死性小肠结肠炎肠道组织病理变化,作用机制可能与双歧杆菌能降低肠道黏膜的TLR4表达及增加TLR2、TLR9表达有关。
Objective: To study the effect of Bifidobacterium on expression of TLR 2,4,9 in neonatal model of necrotizing enterocolitis. Methods: Sprague-Dewley newborn rats were randomly divided into control group, experimental group and treatment group within 12 hours of birth, 12 in each group. The control group was fed by females, and the experimental group was given artificial feeding + hypoxia cold stimulation. The treatment group was treated with artificial feeding + hypoxia cold stimulation and bifidobacterium feeding (2 times / day, 1 × 108cfu / time) . Weighing the body weight and recording the time of day, newborn rats were sacrificed on the third day of the experiment. Pathological changes of intestine of neonatal rats were observed by HE staining. The mRNA expression of TLR2, TLR4 and TLR9 in intestinal mucosa was detected by RT-PCR. The body weight of the three groups were statistically significant, the body weight of the control group was higher than that of the experimental group and the treatment group, and the body weight of the treatment group was higher than that of the experimental group (P <0.05); The pathological score of the experimental group was higher than that of the control group and the treatment group (P <0.05). The relative expression of TLR4 mRNA in the experimental group was higher than that in the control group and the treatment group (P <0.05), and the relative expression of TLR2 mRNA and TLR9 mRNA in the treatment group was higher than that in the experimental group , While the relative expression of TLR4 mRNA was lower than that of the experimental group. The relative mRNA expression levels of the three receptors in both groups were statistically significant (P <0.05). Conclusion: Bifidobacterium can reduce pathological changes of intestinal tissue of necrotizing enterocolitis in neonatal rats. The possible mechanism is that Bifidobacterium can reduce the expression of TLR4 and increase the expressions of TLR2 and TLR9 in intestinal mucosa.