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目的 了解新鲜羊膜移植在早期角膜碱烧伤治疗中抑制炎症和促上皮愈合的作用 ,并与保存羊膜作比较.方法 以 48只新西兰大白兔制作角膜碱烧伤模型 ,2 4h后设立新鲜羊膜移植组、保存羊膜移植组和对照组.术后 14、30 d取材角膜进行组织病理学检查 ,以计算机图像分析系统测量其多形核白细胞含量 ,扫描电镜和透射电镜观察角膜超微结构改变.结果 新鲜羊膜移植组角膜组织中多形核白细胞含量低于保存羊膜移植组 ,二者均低于对照组 (P“,”Objective To understand the effects of fresh amnion tran splomtation on restraining PMN and promoting epithelial healing in the treatment of early corneal alkali burn.Methods Corneal alkali burn model of New Zealand white rabbits was made. The rabbits were divided into fresh amnion transplantation group (fAMT),preserved amnion transplantation group (pAMT) and control group (C).On the 14th day and 30th day after operation, the contents of polymorphonuclear leukocyte (PMN) in corneas by using computer image analysis system were measured. Corneal ultrastructural morphology was observed by scanning electron microscope and transmission electron microscope.Results The contents of PMN in fAMT were lower than those in pAMT,and both were lower than those in C.Conclusion Fresh amnion transplantation has stronger effect on restraining inflammatary infiltration and promoting corneal epithelial healing than preserved amnion transplantation.