论文部分内容阅读
目的和方法 :为探讨急性髓系白血病细胞外基质纤维组织的动态变化对白血病细胞生长可能产生的影响。应用倒置显微镜及病理特殊染色 (Gomori&Masson)观察 15例AML骨髓细胞长期培养 (LTBMC)贴壁层融合及网状纤维、胶原纤维含量 (半定量法 )的变化。结果 :(1) 8/ 15例获独立生长的AML(AMLsm)在培养的 1～ 8周网状纤维含量显著低于正常对照和未获独立生长的AML(AMLnsm) (P <0 0 5 )。而AMLsm的胶原纤维含量在培养的 1～ 6周显著高于正常对照和AMLnsm(P <0 0 5 ) ;(2 )胶原丰富区域见大量造血细胞 ,而网状纤维密集区造血细胞稀少。 (3)培养过程中贴壁层呈大部分融合的仅见于 3例AMLsm。结论 :适量的纤维组织是造血细胞生长所必需的 ,而其组成比例异常可能在白血病及其它一些恶性血液病的发生上起一定的作用
Objective and Methods: To investigate the effect of dynamic changes of extracellular matrix fibrosis in acute myeloid leukemia on leukemia cell growth. Inverted microscopy and pathological special staining (Gomori & Masson) were used to observe the changes of adherent layer fusion, reticular fiber and collagen fiber content (semi-quantitative) in 15 AML bone marrow cells long-term culture (LTBMC). Results: (1) AML (AMLsmm) with 8/15 independent growths at 1 ~ 8 weeks after culture was significantly lower than that of AML (AMLnsm) (P <0 05) . However, collagen fiber content of AMLsm was significantly higher than that of normal control and AMLnsm (P <0 05) at 1 to 6 weeks of culture. (2) A large number of hematopoietic cells were seen in collagen-rich area, while few hematopoietic cells were found in reticular fiber-dense area. (3) Most of the adherent layers in the process of culture were found only in 3 cases of AMLsm. CONCLUSIONS: An adequate amount of fibrous tissue is necessary for hematopoietic cell growth and an abnormal proportion of its composition may play a role in the development of leukemia and other hematologic malignancies