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目的研究白藜芦醇对高能量饮食大鼠胰岛素敏感性的影响。方法清洁级雄性Wistar大鼠35只,随机分为对照组(正常Cont和高糖高脂对照组HFG)和低、中、高白藜芦醇剂量组(HFG+R1,2,3)。12W后分别进行口服葡萄糖耐量试验和胰岛素耐量试验。13W末,断头处死,测空腹血糖和血胰岛素,计算稳态评估胰岛素抵抗指数(HOMA-IR指数)。实时荧光定量PCR技术检测主动脉磷脂酰肌醇3激酶p85αmRNA水平变化。结果与高糖高脂组比,白藜芦醇显著降低大鼠体重,调节血脂(P<0.05)。白藜芦醇还可显著降低大鼠血胰岛素水平和HOMA-IR指数,改善高糖高脂饮食诱导的大鼠葡萄糖耐量和胰岛素耐量异常(P<0.05)。高糖高脂饮食使大鼠p85αmRNA表达降低,中、高剂量的白藜芦醇则可遏制高糖高脂的有害作用(P<0.05)。结论白藜芦醇可提高高糖高脂饮食大鼠胰岛素敏感性,预防胰岛素抵抗的发生,其机制可能与调节主动脉PI3K p85αmRNA水平有关。
Objective To investigate the effect of resveratrol on insulin sensitivity in high-energy diet rats. Methods Thirty-five clean male Wistar rats were randomly divided into control group (normal Cont and high-sugar high-fat control group HFG) and low, medium and high resveratrol dose group (HFG+R1,2,3). Oral glucose tolerance test and insulin tolerance test were performed after 12W. At the end of 13W, the rats were sacrificed by head-to-head, fasting blood glucose and blood insulin were measured, and the steady-state evaluation of insulin resistance index (HOMA-IR index) was calculated. Real-time fluorescence quantitative PCR was used to detect the change of aortic phosphatidylinositol 3-kinase p85α mRNA level. Results Compared with the high-sugar high-fat diet group, resveratrol significantly reduced the body weight of rats and regulated blood lipids (P<0.05). Resveratrol also significantly reduced blood insulin levels and HOMA-IR index in rats, and improved glucose tolerance and insulin tolerance in rats induced by high glucose and high fat diet (P<0.05). High-sugar and high-fat diet reduced the expression of p85αmRNA in rats, and medium- and high-dose resveratrol suppressed the harmful effects of high glucose and high fat (P<0.05). Conclusion Resveratrol can increase insulin sensitivity and prevent insulin resistance in rats with high-sugar and high-fat diet. The mechanism may be related to the regulation of mRNA expression of PI3K p85α in aorta.