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目的旨在测定左氟沙星药代动力学。用ＲＰＨＰＬＣ法，以环丙沙星为内标，反相Ｃ１８为分析柱，１０ｍｍｏｌ·Ｌ－１磷酸二氢钾—１０ｍｍｏｌ·Ｌ－１溴化四丁铵—乙腈（４５∶４５∶１０）为流动相，磷酸调至ｐＨ３０，检测波长２９５ｎｍ，测定血浆和脑脊液中左氟沙星浓度，平均回收率分别为７４７６％和８２４３％，日内日间误差小于５％，最低检测浓度血浆１０μｇ·Ｌ－１，脑脊液６μｇ·Ｌ－１。１０名开颅手术病人单次口服左氟沙星片３００ｍｇ的血液和脑脊液药代动力学特征均符合开放性一室模型。
The purpose is to determine the pharmacokinetics of levofloxacin. RP-HPLC method to ciprofloxacin as an internal standard, reversed-phase C18 column, 10mmol·L-1 potassium dihydrogen phosphate-10mmol·L-1 tetrabutylammonium bromide - acetonitrile (45:45:10 ) As the mobile phase, the pH value of phosphoric acid was adjusted to 0, the detection wavelength of 295nm, the determination of plasma and cerebrospinal fluid concentrations of levofloxacin, the average recovery was 74 76% and 82 43%, intraday error was less than 5% The minimum detectable concentration of plasma 10μg · L-1, CSF 6μg · L-1.10 craniotomy patients with a single oral levofloxacin tablets 300mg of blood and cerebrospinal fluid pharmacokinetic characteristics are in line with the open-studio model.