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利用栽培花生中3个类型各一分品种种子的胚根、胚轴、胚芽、子叶等外植体进行愈伤组织的诱导和分化。结果表明:所有的外植体均能产生愈伤组织。除胚根外,其它3种外植体的愈伤组织均能分化出不定芽,尤以胚芽的愈伤组织分化能力为最强。用MS+2mg/1NAA+1mg/1 6-BA+2mg/1 2.4-D与MS+1mg/1 NAA+3mg/1 6-BA培养基配合使用。3个品种分化有效值平均为3.65,高者达5.3。幻苗在MS+0.4mg/l IBA培养基上能产生正常的根,长成健壮的再生植株。
Induction and differentiation of callus were carried out by using explants such as radicle, hypocotyl, germ and cotyledon of three varieties of peanut. The results showed that all the explants could produce callus. In addition to the radicle, the callus of the other three explants all differentiated into adventitious buds, especially the embryogenic callus differentiation ability strongest. Use with MS + 2 mg / 1 NAA + 1 mg / 1 6-BA + 2 mg / 1 2.4-D with MS + 1 mg / 1 NAA + 3 mg / 1 6-BA medium. The average effective differentiation value of three cultivars was 3.65 and the highest one reached 5.3. Miao Miao in MS + 0.4mg / l IBA medium to produce normal roots, grow into robust regenerated plants.