用高效液相色谱法测定 30 μm、6 0 μm PHT作用下培养神经元细胞内及分泌到细胞外谷氨酸的含量。并同时用放射酶测定法测定细胞内谷氨酸脱羧酶及 FA- 2 /AM法测细胞内游离 Ca2 + 的变化情况。结果示苯妥英钠作用下细胞内谷氨酸含量及谷氨酸脱羧酶活性无变化 ,细胞内 Ca2 +一过性降低 ,细胞分泌谷氨酸水平明显降低。说明苯妥英钠可能是通过降低细胞内Ca2 +浓度 ,减少细胞对兴奋性神经递质谷氨酸的分泌而发挥药理作用。 The content of glutamate in cultured neurons and secreted extracellularly by 30 μm, 60 μm PHT was determined by high performance liquid chromatography. At the same time, the changes of intracellular free Ca2 + by intracellular glutamate decarboxylase and FA-2 / AM assay were determined by radioimmunoassay. The results showed that phenytoin sodium intracellular glutamate content and glutamic acid decarboxylase activity was unchanged, a transient decrease in intracellular Ca2 +, cell secretion of glutamate levels were significantly lower. That phenytoin sodium may reduce the intracellular Ca2 concentration, reduce the secretion of neurotransmitter glutamate cells play a pharmacological role.