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本文参照国外文献加以改进建立了AT Ⅲ分离纯化方法,整个流程分为聚乙二醇4000沉淀蛋白,肝素—Sepharose 4B亲和层析以及DEAE—Sephadex A50离子交换三个步骤.分离出AT Ⅲ用发色三肽底物法测定
In this paper, reference to foreign literature to be improved to establish the AT Ⅲ separation and purification methods, the entire process is divided into polyethylene glycol 4000 precipitation protein, heparin-Sepharose 4B affinity chromatography and DEAE-Sephadex A50 ion exchange three steps. Chromogenic tripeptide substrate assay