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目的观察某学院学员长途奔袭训练对肾脏功能的影响。方法受试学员211名,分别在100 km长途奔袭训练前、后30 min内留取尿液进行尿干化学分析和显微镜检查,尿蛋白阳性标本另行十二烷基磺酸钠-琼脂糖凝胶电泳(SDS-AGE)非浓缩尿蛋白分析,并对训练后尿蛋白阳性学员进行复检。结果长途奔袭训练后尿蛋白阳性率19.0%、酮体阳性率55.5%、血红蛋白阳性率24.6%、镜下血尿阳性率9.5%,均显著高于长途奔袭训练前(P<0.01)。电泳分析:单纯白蛋白尿89.6%(43/48),肾小球型蛋白尿4.2%(2/48)、混合性蛋白尿6.2%(3/48)。结论长途奔袭训练致肾脏损伤的发生率较高,尿常规筛查对早期发现和预防肾脏损伤具有重要意义,SDS-AGE非浓缩尿蛋白电泳分析有助于进一步了解肾脏损伤的部位和分型。
Objective To observe the impact of long-distance raiding training on the renal function of a college student. Methods Totally 211 students were enrolled in this study. The urine samples were collected for urine chemical analysis and microscopic examination within 30 min before and after 100 km long-range raiding training. Urine protein positive samples were treated with sodium dodecyl sulfate-agarose gel Electrophoresis (SDS-AGE) non-concentrated urine protein analysis, and after training urinary protein positive students re-examination. Results The positive rate of urine protein was 19.0%, ketone body positive rate was 55.5%, hemoglobin positive rate was 24.6%, microscopic hematuria positive rate was 9.5% after long-distance raiding training, which were all significantly higher than long-distance raiding training before training (P <0.01). Electrophoretic analysis: simple albuminuria 89.6% (43/48), glomerular proteinuria 4.2% (2/48), mixed proteinuria 6.2% (3/48). Conclusion The incidence of renal injury caused by long-distance raiding training is high. Urinary routine screening is of great significance for the early detection and prevention of kidney damage. SDS-AGE non-concentrated urine protein electrophoresis analysis can help further understand the location and classification of renal injury.