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为阐明桂花PPO基因在其代谢活动及经济性状形成过程中可能发挥的作用,本研究综合运用RT-PCR、RACE、生物信息学和荧光实时定量方法,从桂花花瓣中克隆出了多酚氧化酶(PPO)基因,并证实本实验所克隆的基因(长1 866 bp)是PPO基因家族的成员之一;荧光实时定量分析其时空表达发现PPO基因在幼根中表达量最高,花瓣、花梗中较低,当年生革质化叶片中表达量最低,而且PPO基因在开花过程中的表达谱变化趋势呈“高-低-高”。综上所述,桂花PPO基因的时空表达谱揭示了该基因的表达水平与器官的发育阶段、组织类型密切相关。
In order to elucidate the possible role of PPO gene in the process of its metabolic activity and economic traits, we used the RT-PCR, RACE, bioinformatics and real-time fluorescence quantitative methods to clone the polyphenol oxidase (PPO) gene and confirmed that the cloned gene (1 866 bp in length) is a member of the PPO gene family. Real-time quantitative analysis of its expression in time and space revealed that PPO gene had the highest expression level in young root, The expression level of PPO gene in the leaves of the grafted leaves was the lowest, and the expression trend of the PPO gene was “high-low-high” in flowering. In summary, the temporal-spatial expression profile of the Osmanthus fragrans PPO gene reveals that the gene expression level is closely related to the organ development stage and the type of tissue.