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为寻找新型有效的ＡⅡ拮抗剂，本实验应用细胞计数、￣３Ｈ－ＴｄＲ掺入、逆转录─聚合酶链反应及放射免疫实验方法，研究了中药单体７６４－３对卒中易感型自发性高血压大鼠（ＳＨＲｓｐ）及其对照（ＷＫＹ大鼠）主动脉平滑肌细胞（ＡｓＭＣ）增殖的影响及其作用机制。结果表明：７６４－３可显著抑制ＡＳＭＣ增殖，并有剂量依赖性。２０ｍｇ／Ｌ的７６４－３可极显著地抑制ＡＳＭＣ上ＡⅡ受体（ＡＴ＿１）的ｍＫＮＡ表达（ＳＨＲｓｐ下降７６．３％，ＷＫＹ大鼠下降６１．６％），并可降低ＳＨＲｓｐＡＳＭＣ内ＡⅡ含量（下降２０％）。推测７６４－３对ＡＳＭＣ增殖的抑制作用可能部分地是由于降低了ＡＴ＿１受体的基固表达引起。比较其对两种大鼠ＡＳＭＣ的抑制效率，发现它对ＳＨＲｓｐ的ＡＳＭＣ的作用更强。
In order to find a new and effective AII antagonist, we used the cell counting, 3H-TdR incorporation, reverse transcription-polymerase chain reaction and radioimmunoassay to study the effect of traditional Chinese medicine 764-3 on stroke-prone spontaneous Effect and mechanism of proliferation of aortic smooth muscle cells (AsMC) in hypertensive rats (SHRsp) and its control (WKY rats). The results showed that 764-3 significantly inhibited the proliferation of ASMC in a dose-dependent manner. 20mg / L 764-3 could significantly inhibit the expression of AⅡ receptor (AT_1) mKNA in ASMC (76.3% in SHRsp and 61.6% in WKY), and decreased the content of AⅡ in SHRspASMC 20%). It is speculated that the inhibitory effect of 764-3 on ASMC proliferation may be due, in part, to the reduction of the basal expression of the AT 1 receptor. Compared with the inhibitory effect on ASMC of the two kinds of rats, we found that ASMC is more effective on SHRsp.