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为提高肺结核病原学的诊断水平,我们采用聚合酶链反应(PCR)技术检测了36例肺结核患者痰标本中的结核菌,并同涂片法、培养法检查结核菌及与非结核患者进行对照分析研究,以评价PCR诊断肺结核的临床价值。 1 对象与方法①研究对象:36例肺结核患者均为本院呼吸内科及门诊患者。诊断系根据临床表现、胸部X线、细菌学或病理学检查,并结合抗结核治疗有效而确诊。20例非结核患者亦取自本院患者,其中肺癌9例,肺炎5例,慢性阻塞性肺病和支气管扩张各3例。②标本收集:晨起清洁口腔后,深部咳出2～3口痰,留入无菌容器中。每份标本同时分送涂片找抗酸杆菌、结核菌培养及PCR
In order to improve the diagnosis of pulmonary tuberculosis, we used polymerase chain reaction (PCR) to detect tuberculosis in sputum from 36 cases of pulmonary tuberculosis patients, and compared with smear method, culture method to detect tuberculosis and non-tuberculosis patients Analysis, to evaluate the clinical value of PCR diagnosis of pulmonary tuberculosis. 1 objects and methods ① research object: 36 cases of pulmonary tuberculosis patients were hospital respiratory medicine and outpatients. Diagnosis based on clinical manifestations, chest X-ray, bacteriological or pathological examination, combined with anti-TB treatment is effective and confirmed. Twenty patients with non-tuberculosis were also recruited from our hospital, including 9 cases of lung cancer, 5 cases of pneumonia, 3 cases of chronic obstructive pulmonary disease and 3 cases of bronchiectasis. ② collection of specimens: morning after cleaning the mouth, deep cough 2 to 3 phlegm, stay in sterile containers. At the same time each specimen was sent to smear acid-fast bacilli, tuberculosis culture and PCR