The design of enterprise network topology is in fact a multi-object nonlinear programming problem. In this paper, distance, traffic distribution and transmission delay are chosen as the important factors to be considered in the subnetwork partition of the network topology design. A mathematical model is presented and The Genetic Algorithm is used to solve the optimization object function. The application results demonstrate that the method can well solve the problem of subnetwork partition. The design of enterprise network topology is in fact a multi-object nonlinear programming problem. In this paper, distance, traffic distribution and transmission delay are chosen as the important factors to be considered in the subnetwork partition of the network topology design. A mathematical model is presented and The Genetic Algorithm is used to solve the optimization object function. The application results demonstrate that the method can well solve the problem of subnetwork partition.