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土曲霉植酸酶催化植酸水解得到1,2,6-三磷酸肌醇(Ins(1,2,6)P3)。在K3[Fe(CN)6]体系中,Ins(1,2,6)P3还原能力很弱。采用活性氧(ROS)试剂盒测定ROS含量,当浓度为0.4mg/mL时,Ins(1,2,6)P3对化学模拟体系及小鼠血清中活性氧的抑制率分别为90.78%、84.18%。在高浓度(20mg/mL)时,Ins(1,2,6)P3对大鼠红细胞氧化自溶血具有促进作用;低浓度(2mg/mL)时具有明显的抑制作用。小鼠腹腔连续注射Ins(1,2,6)P3(100mg/kg·d)12d后,与对照组比较,血清抗活性氧显著提高。
Aspergillus terreus phytase catalyzes phytic acid hydrolysis to give 1,2,6 (P3 (1,2,6) P3). In K3 [Fe (CN) 6] system, Ins (1, 2, 6) P3 has a weak reducing ability. ROS concentration was measured by reactive oxygen species (ROS) kit. The inhibitory rates of Ins (1,2,6) P3 on the chemical simulated system and the active oxygen in the serum of mice were 90.78% and 84.18 %. At high concentration (20 mg / mL), Ins (1, 2, 6) P3 promoted oxidative hemolysis of erythrocytes in rats, and significantly inhibited at low concentration of 2 mg / mL. Serum anti-reactive oxygen was significantly increased in mice injected with Ins (1,2,6) P3 (100mg / kg · d) for 12 days.