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1986～1988年我们应用绵羊棘球蚴囊壁冰冻切片抗原作间接荧光抗体试验分别检测了13例包虫病人血清和滤纸干血滴,结果均为阳性,阳性率为100％(13/13);24例健康人血清和滤纸干血滴的阴性率分别为95.8％(23/24)和100％(24/24)。本方法敏感性和特异性较高,可节省抗原材料,采血简便易行,是包虫病较为理想的辅助诊断方法。为了研究其实用价值,我们又检测了甘肃省天祝县251人,漳县242人和会宁县683人,阳性率分别为4.78％(12/251),5.37％(13/242)和1.17％(8/683),其结果与间接血凝试验的结果(阳性率分别为4.78％,4.96％和0.9%)之间无显著性差异(P>0.05)。作者认为将该方法用于包虫病流行区人群的血清流行病学调查,可反映人群包虫病的感染率。
From 1986 to 1988, we used the indirect immunofluorescence assay of frozen section antigen of hydatid cyst of sheep to detect the serum and filter paper dry blood droplets of 13 hydatid patients respectively, the result was positive, the positive rate was 100% (13/13 ). The negative rates of serum and filter blood dry-blood droplets in 24 healthy volunteers were 95.8% (23/24) and 100% (24/24), respectively. The method has high sensitivity and specificity, can save antigenic material, is convenient and easy to collect blood, and is an ideal auxiliary diagnosis method of hydatid disease. In order to study its practical value, we examined 251 people in Tianzhu County of Gansu Province, 242 people in Zhang County and 683 people in Huining County. The positive rates were 4.78% (12/251), 5.37% (13/242) and 1.17% (8/683). There was no significant difference between the results and indirect hemagglutination test (positive rates were 4.78%, 4.96% and 0.9% respectively) (P> 0.05). The author believes that this method will be applied to the epidemiological investigation of serogroups in echinococcosis endemic areas, which can reflect the infection rate of human echinococcosis.