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青霉菌 (Penicilliumsp菌株 )发酵液经 2 0 %～ 70 %硫酸铵分级沉淀 ,DEAE -Cellulose离子交换层析 ,SephadexG - 10 0凝胶过滤层析 ,得到聚丙烯酰胺凝胶电泳均一的壳聚糖酶 ,该壳聚糖酶具有很强的底物专一性
The Penicillium sp. Broth was fractionated by 20% ~ 70% ammonium sulfate, DEAE-Cellulose ion exchange chromatography and Sephadex G-10 gel filtration chromatography to obtain uniform polyacrylamide gel electrophoresis Enzymes, which have strong substrate specificity