论文部分内容阅读
为检测狼疮样肾炎小鼠脾、肾组织中钙调磷酸酶 (CaN )活性 ,以探讨其与肾炎小鼠肾组织RANTES表达及肾组织病理改变的关系。采用慢性移植物抗宿主小鼠 (GVHD )狼疮样肾炎模型 ,CaN活性检测采用发色底物法。结果 :(1) 12周模型组肾小球硬化指数及小管间质损伤总积分显著高于 8周模型组 (P <0 0 1) ;(2 ) 12周模型组RANTES蛋白及mRNA表达显著高于 8周模型组 (P <0 0 1) ,FK5 0 6显著抑制狼疮样肾炎小鼠肾组织RANTES蛋白及mRNA表达 ;(3)模型组脾、肾组织中CaN活性显著增强 ,与正常对照组脾、肾组织CaN活性有显著差异 (P <0 0 1) ,FK5 0 6组中CaN活性显著下降 (P <0 0 5 ) ;(4 )狼疮样肾炎小鼠脾组织中CaN活性与血清抗dsDNA抗体水平呈正相关关系 (r1=0 92 5 ,P <0 0 1) ;(5 )狼疮样肾炎小鼠肾组织中CaN活性与肾小球硬化指数及小管间质损伤总积分呈正相关关系 (r2 =0 6 2 6 ,P <0 0 1;r3 =0 772 ,P <0 0 5 ) ,与RANTES蛋白阳性小管数呈正相关关系 (r4=0 6 2 5 ,P <0 0 5 ) ,狼疮样肾炎小鼠肾组织中CaN活性与RANTESmRNA表达呈正相关关系 (r5=0 714 ,P <0 0 5 )。表明狼疮样肾炎小鼠脾细胞存在CaN异常活化 ,肾组织中异常活化的CaN参与了肾脏病理损伤
To detect calcineurin (CaN) activity in spleen and kidney of lupus-like nephritis mice and to explore the relationship between the expression of RANTES and the pathological changes of renal tissue in nephritis mice. Chronic graft-versus-host mice (GVHD) were used in the model of lupus-like nephritis. CaN activity assay was performed using the chromogenic substrate method. Results: (1) The total score of glomerular sclerosis index and tubulointerstitial injury in model group at 12 weeks was significantly higher than that in 8-week model group (P <0.01). (2) The expression of RANTES protein and mRNA in 12-week model group was significantly higher In the model group at 8 weeks (P <0.01), FK5 0 6 significantly inhibited the expression of RANTES protein and mRNA in the kidney of lupus-like nephritis mice; (3) The activity of CaN in the model group was significantly increased compared with the normal control group There were significant differences in CaN activity in spleen and kidney (P <0.01), CaN activity in FK5 0 6 group (P <0.05); (4) CaN activity in spleen tissue of lupus-like nephritis mice was significantly correlated with serum anti- dsDNA antibody level (r1 = 0 92 5, P <0.01); (5) The activity of CaN in renal tissue of lupus-like nephritis mice was positively correlated with the glomerular sclerosis index and the total score of tubulointerstitial injury r2 = 0 6 2 6, P <0.01; r3 = 0 772, P <0 05), and positively correlated with RANTES positive tubules (r4 = 0 625, P <0 05) There was a positive correlation between CaN activity and RANTES mRNA expression in the kidney of nephritis-like nephropathy mice (r5 = 0 714, P <0 05). It showed that there was CaN abnormal activation in splenocytes of lupus-like nephritis mice and abnormal activation of CaN in renal tissues was involved in renal pathological injury