论文部分内容阅读
目的 观察膳食总热量正常、脂肪含量长期增高饲养对正常大鼠葡萄糖刺激的胰岛素分泌的影响。方法 8周龄雄性SD大鼠 1 9只 ,分为正常饲养组 (NC ,n =1 0 )和高脂饲养组 (HF ,n =9)。两组大鼠每日摄食总热量相等 ,但饲料中脂肪占总热量 (31 0kJ/d)的百分比不同 (NC组脂肪占 1 3 % ,HF组脂肪占 59% )。观察大鼠每月体重、空腹血糖 (FBG)、空腹胰岛素 (FINS)、空腹游离脂肪酸 (FFFA)的变化。饲养至 36周龄 ,作静脉葡萄糖耐量试验 (IVGTT) ,观察两组大鼠葡萄糖刺激的胰岛素分泌 ,尤其是胰岛素分泌的第一时相的差异。结果 高脂饲养后大鼠出现明显的腹型肥胖 (内脏脂肪占体重百分比分别为 :HF组 1 0 .7% ,NC组 5 .3 % ,P <0 .0 0 1 ) ,血FFFA升高〔HF组 (1 .60± 0 .36)mmol/L ,NC组 (0 .59± 0 .1 2 )mmol/L ,P <0 .0 0 1〕。 36周龄时 ,IVGTT中 5、 1 0、 30min血糖 (BG)HF组显著高于NC组〔5min :(1 7.3± 0 .7)mmol/Lvs(1 6 .0± 0 .7)mmol/L ,P <0 .0 5 ;1 0min :(1 4 .6± 0 .8)mmol/Lvs (1 2 .7± 1 .0 )mmol/L ,P <0 .0 0 1 ;30min :(9.4± 1 .6)mmol/Lvs (6 .6± 1 .4)mmol/L ,P <0 .0 1〕。 5、1 0min血胰岛素HF组显著低于NC组〔5min :(734 .7± 1 .5) pmol/Lvs (1 938.4± 1 .4)pmol/L ,P <0 .0
Objective To observe the effects of dietary total calories and long-term high fat content on glucose-stimulated insulin secretion in normal rats. Methods Nineteen male Sprague-Dawley rats aged 8 weeks were divided into normal group (NC, n = 10) and HF group (HF, n = 9). The total daily calorie intake was equal in both groups, but the percentages of fat in the diet (31 0 kJ / d) were different (33% in the NC group and 59% in the HF group). The changes of body weight, fasting blood glucose (FBG), fasting insulin (FINS) and fasting free fatty acid (FFFA) in rats were observed. The animals were fed to 36 weeks of age for intravenous glucose tolerance test (IVGTT). The difference of glucose-stimulated insulin secretion, especially the first phase of insulin secretion, was observed in both groups. Results Obvious abdominal obesity was observed in rats fed high fat diet (visceral fat accounted for 10.7% of body weight, 7.3% of NC group, P <0.01), and elevated blood FFFA (HF group (1.60 ± 0.36) mmol / L, NC group (0 .59 ± 0.12 mmol / L, P <0.001). At 36 weeks of age, blood glucose (BG) at 5,1,0 and 30 minutes in IVGTT was significantly higher in the HF group than in the NC group [5 min: (1 7.3 ± 0 .7) mmol / L vs (16 ± 0 · 7) mmol / L, P <0.05; 10 min: (14.6 ± 0.8) mmol / L vs (12.7 ± 1.0) mmol / L, P <0.01; 30min :( 9.4 ± 1 .6) mmol / L vs (6.6 ± 1.4 mmol / L, P <0.01). 5,1 0min blood insulin group HF group was significantly lower than the NC group [5min: (734.7 ± 1.5] pmol / Lvs (1 938.4 ± 1. 4 pmol / L, P <0 0