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目的 :探讨胃癌组织中c -erbB - 2和p5 3基因的表达与胃癌发生和浸润转移的关系。方法 :应用荧光原位杂交技术对 5 5例胃癌的常规石蜡标本进行检测。结果 :c -erbB - 2和 p5 3基因表达的阳性率分别为 36 .6 %和 45 .45 %。其中在肠型和弥漫型胃癌中 ,c -erbB - 2和 p5 3阳性率分别为 5 1.6 15和 16 .6 7% ,p5 3阳性率分别为 2 5 .81%和 70 .83% ,两种基因在两型间的差异有显著性意义 (P <0 .0 5 )。c-erbB - 2和p5 3基因表达与胃的组织分级有关 (分别为P <0 .0 5及P <0 .0 1)c -erbB - 2和 p5 3基因表达与胃癌的浸润深度有相关性 (分别为P <0 .0 1及 <0 .0 5 )。c -erbB - 2基因表达与胃癌的淋巴结内转移有显著性意义 (P <0 .0 5 )。结论 :c -rebB - 2和 p5 3基因有助于确定胃癌的生物学行为。
Objective: To investigate the relationship between the expression of c-erbB-2 and p53 gene and the occurrence, invasion and metastasis of gastric cancer. Methods : Fifty-five cases of normal paraffin specimens from gastric cancer were detected by fluorescence in situ hybridization. Results: The positive rates of c-erbB-2 and p53 gene expression were 36.6% and 45.4%, respectively. Among the intestinal and diffuse gastric cancers, the positive rates of c-erbB-2 and p53 were 51.615 and 16.6%, respectively, and the p53 positive rates were 25.81% and 70.83% respectively. There was a significant difference between the two types of genes (P < 0.05). The expression of c-erbB-2 and p53 genes was correlated with the histological grade of the stomach (P < 0.05 and P <0.01, respectively). The expression of c-erbB-2 and p53 genes was correlated with the depth of invasion of gastric cancer. Sex (P <0 1 and <0 5, respectively). The c-erbB-2 gene expression was significantly associated with lymph node metastasis in gastric cancer (P < 0.05). Conclusion: The c-rebB-2 and p53 genes help to determine the biological behavior of gastric cancer.