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目的构建水溶性的Cd Te纳米晶荧光探针,研究其与克伦特罗抗体之间的相互关系。方法以L-半胱胺作为修饰剂,在水溶液中合成稳定的Cd Te纳米晶。用紫外可见吸收光谱和荧光发射光谱、TEM和XRD图谱进行表征。考察制备条件对Cd Te纳米晶荧光性能的影响。结果当Cd2+:HTe-:L-Cysteamine的摩尔比为2∶1∶4时,反应体系的p H为5.6,在100℃水浴中回流3 h,其荧光量子产率可达54.68%,半峰宽仅为48 nm。结论此方法不但缩短了Cd Te纳米晶的制备时间,而且性能稳定,在EDC和NHS共价偶联的作用下,实现了对克伦特罗抗体的连接,为荧光免疫分析方法提供了性能优良的荧光探针。
Objective To construct a water-soluble CdTe nanocrystal fluorescent probe and study its relationship with clenbuterol antibody. Methods L-cysteamine was used as modifier to synthesize stable Cd Te nanocrystals in aqueous solution. UV-visible absorption spectra and fluorescence emission spectra, TEM and XRD patterns were characterized. Effects of Preparation Conditions on Fluorescence Properties of Cd Te Nanocrystals. Results When the molar ratio of Cd2 +: HTe-: L-Cysteamine was 2:1: 4, the p H of the reaction system was 5.6 and the fluorescence quantum yield reached 54.68% when refluxed at 100 ℃ for 3 h. The half peak The width is only 48 nm. Conclusions This method not only shortens the preparation time of Cd Te nanocrystals, but also has stable properties. Under the covalent coupling of EDC and NHS, the cleavage of antibodies to clenbuterol is achieved, and the fluorescence immunoassay method provides excellent performance Fluorescent probe.