论文部分内容阅读
目的建立癃闭舒片中补骨脂素和异补骨脂素的定量方法。方法对国内3个企业生产的6个批次的癃闭舒片,采用HPLC法检测,Agilent TC-C18(2)色谱柱(250 mm×4.6 mm,5μm),流动相是甲醇-水(43?57),体积流量1.0 m L/min,检测波长为246 nm,柱温30℃。结果补骨脂素回归方程为:Y=84.44X+9.994,r=0.999 9(n=12)。异补骨脂素回归方程为:Y=82.434X+8.8952,r=0.999 9(n=12)。补骨脂素和异补骨脂素的线性范围均在4.04~151.5μg。结论本方法操作简便、准确、专属性强、稳定,可用于癃闭舒片中补骨脂素和异补骨脂素的含量测定。
Objective To establish a method for quantitative determination of psoralen and isopsoralen in Tuanhuashu Tablets. Methods Six batches of Shuanhu Shu Tablets produced by 3 domestic enterprises were detected by HPLC. The mobile phase was Agilent TC-C18 (2) column (250 mm × 4.6 mm, 5 μm) with methanol-water ? 57), volume flow 1.0 m L / min, detection wavelength of 246 nm, column temperature 30 ℃. Results Psoralen regression equation was: Y = 84.44X + 9.994, r = 0.999 9 (n = 12). The isopsoralen regression equation was: Y = 82.434X + 8.8952, r = 0.999 9 (n = 12). The linear range of psoralen and isopsoralen was 4.04 ~ 151.5μg. Conclusion The method is simple, accurate, specific and stable. It can be used to determine the content of psoralen and isopsoralen in Tuanhuashu Tablets.