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运用硅胶、sephadexLH-20等柱色谱,从木蹄层孔菌子实体的乙醇提取物分离得到12个化合物,通过单体的理化性质、NMR和MS技术鉴定单体的结构为3-十六碳酸酯-7,22-二烯麦角甾醇(1),十八烷酸(2),棕榈酸(3),7,22-二烯麦角甾-3-酮(4),麦角甾-7,22-二烯-3-醇(5),5,8-过氧麦角甾-6,22-二烯-3-醇(6),3,3-二甲氧基-7,22-二烯麦角烷(7),28-乙酰白桦脂醇(8),白桦脂醇(9),β-羟基十八烷酸(10),9,10-二羟基十八烷酸(11),瑞香素(12)。采用AlamarBlue法检测单体化合物对人肺癌细胞NCI-H460和人胃癌细胞SGC-7901的抑制活性。结果表明,化合物4对NCI-H460细胞株的抑制活性最高,化合物9对SGC-7901的抑制活性最高。
Twelve compounds were isolated from the ethanol extracts of Mytiapsis stomatalus by silica gel and sephadex LH-20 column chromatography. The structures of the monomers were identified as 3-hexadecanoate by physical and chemical properties, NMR and MS techniques (2), palmitic acid (3), 7,22-dienone ergosterol-3-one (4), ergostat-7,22- 3-ol (6), 3,3-dimethoxy-7,22-dienylglucose (7) 28-acetyl betulin (8), betulin (9), β-hydroxyoctadecanoic acid (10), 9,10- dihydroxyoctadecanoic acid (11), daphnetin ). The inhibitory activities of monomeric compounds on human lung cancer cell line NCI-H460 and human gastric cancer cell line SGC-7901 were determined by AlamarBlue assay. The results showed that compound 4 had the highest inhibitory activity on NCI-H460 cell line, and compound 9 had the highest inhibitory activity on SGC-7901.