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为了探讨逆转录病毒介导的CDglyTK自杀基因系统对K562细胞的体内外杀伤作用,将逆转录病毒介导的CDglyTK自杀基因转染入K562细胞,体外实验用MTT法观察5-氟胞嘧啶/丙氧鸟苷(5-fluorocytosine/ganciclovir,5-FC/GCV)对K562/CDglyTK细胞的生长抑制率。体内实验时将K562/CDglyTK细胞和K562细胞接种于裸鼠皮下,使用GCV和5-FC后,观察裸鼠肿瘤体积的变化及裸鼠的生存率。体外实验表明,GCV联合5-FC对K562/CDglyTK细胞具有明显的杀伤作用;体内实验结果显示,皮下注射K562细胞和K562/CDglyTK细胞后小鼠成瘤率无明显区别;使用5-FC/GCV可明显抑制裸鼠体内的肿瘤形成;经5-FC/GCV治疗后K562/CDglyTK组的肿瘤体积较对照组明显缩小,裸鼠生存率也较对照组明显提高。结论:双自杀基因在体内外对K562细胞均有杀伤作用。
In order to investigate the killing effect of retrovirus-mediated CDglyTK suicide gene system on K562 cells in vitro and in vivo, retrovirus-mediated CDglyTK suicide gene was transfected into K562 cells in vitro. MTT assay was used to observe the effect of 5-fluorocytosine / Growth inhibitory rate of 5-fluorocytosine / ganciclovir (5-FC / GCV) on K562 / CDglyTK cells. In vivo, K562 / CDglyTK cells and K562 cells were inoculated subcutaneously in nude mice. The changes of tumor volume and the survival rate of nude mice were observed after using GCV and 5-FC. In vitro experiments showed that GCV combined with 5-FC had obvious cytotoxicity on K562 / CDglyTK cells. The in vivo results showed that there was no significant difference in tumor formation rate between K562 cells and K562 / CDglyTK cells after inoculation with 5-FC / GCV The tumor volume in K562 / CDglyTK group was significantly reduced compared with that in control group after 5-FC / GCV treatment, and the survival rate of nude mice was significantly higher than that in control group. Conclusion: Double suicide genes can kill K562 cells both in vitro and in vivo.