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目的:建立一种反相HPLC法监测人全血中环孢素浓度的方法。方法:采用反相C_(18)色谱柱;柱温:70℃;流动相:乙腈-甲醇-水=160:30:15;流速:0.8ml·min~(-1);检测波长:210nm。结果:在15～1 600ng·ml~(-1)范围内,环孢素与内标的峰高比与环孢素浓度有良好的线性关系(r=0.999 3),最低检测限为5 ng·ml~(-1),高、中、低3种浓度平均相对回收率为96.0%～98.3%;日内RSD为1.2%～3.4%(n=5),日间RSD为3.4%～4.3%(n=5)。结论:该法简单、准确、经济,可用于环孢素的血药浓度监测。
OBJECTIVE: To establish a reverse phase HPLC method for the determination of ciclosporin concentration in human whole blood. Methods: The reversed phase C_ (18) column was used. The column temperature was 70 ℃. The mobile phase was acetonitrile - methanol - water = 160: 30: 15. The flow rate was 0.8 ml · min -1. Results: In the range of 15 ~ 1 600 ng · ml ~ (-1), the peak height ratio of cyclosporine and internal standard had a good linear relationship with the concentration of cyclosporine (r = 0.999 3), the lowest detection limit was 5 ng · The average relative recovery was 96.0% ~ 98.3% at three levels of high, middle and low. The intraday RSD ranged from 1.2% to 3.4% (n = 5) and the intraday RSD ranged from 3.4% to 4.3% n = 5). Conclusion: The method is simple, accurate and economical and can be used for the monitoring of cyclosporine blood concentration.