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目的探讨Identifiler Plus Kit直接扩增血样的方法,并应用于DNA数据库建设。方法 60份血样分别打孔于8连管中,用56℃ddH2O分别孵育0min、5min、10min、15min,离心弃上清,相应加入Identifiler Plus Kit与Identifiler Direct Kit PCR反应液,电泳并比较检测结果;同一血样按上述方法重复3次,比较检测结果的可重复性;对10000余份血样直接扩增并统计分析其成功率。结果 0min与5min ddH2O孵育的血样Identifiler Plus Kit与Identifiler Direct Kit扩增想比较,分析峰值均衡性及RFU其效果相当;同一份血样检测结果表明具有良好的重复性;对10000余份血样检测完整的遗传图谱成功率98.5%。结论 Identifiler Plus Kit可用于大规模的DNA数据库建设。
Objective To explore the method of direct amplification of blood samples by Identifiler Plus Kit and to construct DNA database. Methods Sixty blood samples were perforated in 8 - tube tubes. The cells were incubated with 56 ℃ ddH2O for 0min, 5min, 10min and 15min, respectively. The supernatant was discarded by centrifugation. Identifiler Plus Kit and Identifiler Direct Kit were added to the reaction mixture. Test results; the same blood samples were repeated three times as above, the repeatability of the test results were compared; more than 10000 blood samples were directly amplified and the statistical analysis of the success rate. Results Compared with the amplification of Identifiler Plus Kit in Identifier Plus Kit incubated with 0min and 5min ddH2O, the analysis of peak balance and RFU showed the same effect. The same blood sample showed good reproducibility; more than 10000 blood samples The complete genetic map 98.5% success rate. Conclusion Identifiler Plus Kit can be used for large-scale DNA database construction.