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该研究采用RACE技术从香蕉中克隆了4条乙醇脱氢酶基因——MaADH1(GenBank No.KM253748)、MaADH2(GenBank No.KM253749)、MaADH3(GenBank No.KM253750)、MaADH4(GenBank No.KM253753),且在核苷酸水平上4条基因与2A基因组中的乙醇脱氢酶同源性较高;遗传进化树分析显示,MaADH2、MaADH3和MaADH4属于乙醇脱氢酶第I类,而MaADH1不属于第I类,也不属于第Ⅲ类。半定量RT-PCR分析显示,4条基因在不同器官中的表达量不同;不同激素、不同非生物胁迫以及生物胁迫处理后4条基因的表达显示,MaADH2受ABA、乙烯、茉莉酸、水杨酸、干旱和涝害诱导表达,最大表达量分别为19.14、428.19、68.21、61.79、53.73和108.43;MaADH3受盐胁迫诱导表达,最大表达量为220.27;MaADH1和MaADH4在不同处理后的表达量变化不明显。研究表明,在香蕉中MaADH2可以作为ABA、乙烯、茉莉酸、水杨酸、干旱和涝害的标记基因,MaADH3可以作为盐害的标记基因。
In this study, four alcohol dehydrogenase genes MaaH1 (GenBank No. KM253748), MaADH2 (GenBank No. KM253749), MaADH3 (GenBank No. KM253750) and MaADH4 (GenBank No. KM253753) were cloned from banana using RACE technique. , And 4 genes at the nucleotide level had higher homology with the alcohol dehydrogenase in 2A genome. The phylogenetic tree analysis showed that MaADH2, MaADH3 and MaADH4 belonged to class I of alcohol dehydrogenase, whereas MaADH1 did not belong to Class I does not belong to class III. Semi-quantitative RT-PCR analysis showed that the four genes expressed differently in different organs. The expression of four genes after different hormones, different abiotic stress and biotic stress treatment showed that MaADH2 was regulated by ABA, ethylene, jasmonic acid, Acid, drought and waterlogging induced expression, the maximum expression was 19.14,428.19,68.21,61.79,53.73 and 108.43; MaADH3 induced by salt stress, the maximum expression was 220.27; MaADH1 and MaADH4 in different treatment changes in expression Not obvious. Studies have shown that MaADH2 can be used as a marker gene for ABA, ethylene, jasmonic acid, salicylic acid, drought and waterlogging in bananas, and MaADH3 can be used as a marker gene for salt damage.