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按常规分子克隆方法将马铃薯中高表达的patatin启动子和乙肝表面抗原基因分别插入双元质粒pBI101成为植物表达载体pPHG9,由前者驱动后者表达;通过农杆菌转化法将乙肝表面抗原基因导入马铃薯并获得再生植株;用ELISA方法检测经PCR鉴定的转基因植株中乙肝表面抗原的含量,对照标准曲线得知转基因马铃薯植株中的乙肝表面抗原含量高达100～174ng/mg可溶性蛋白
According to the conventional molecular cloning method, the patatin promoter and hepatitis B surface antigen gene highly expressed in potato were respectively inserted into the binary plasmid pBI101 to be a plant expression vector pPHG9, which was driven by the former. The gene of hepatitis B surface antigen was introduced into potato by Agrobacterium transformation The regenerated plants were obtained. The content of HBsAg in the transgenic plants identified by PCR was detected by ELISA. The standard curve of HBsAg in the transgenic potato plants was as high as 100-174 ng / mg soluble protein