目的:了解1例新型冠状病毒肺炎(Coronavirus Disease 2019, COVID-19)确诊病例的病原学构成。方法:将1例COVID-19确诊病例的口咽拭子、血清、鼻咽拭子的标本接种到Vero-E6细胞管进行病毒分离，每日观察细胞病变(cytopathic effect, CPE)情况，待CPE达75%以上收获细胞分离物，反复冻融3次后离心取上清液，应用透射电镜观察获取病毒电镜图片，同时提取病毒核酸，采用二代测序技术进行全基因组序列测序。通过序列进化分析，对获得的病毒株进行鉴定分型。结果:本研究从1例COVID-19患者的鼻咽拭子中分离到1株新型冠状病毒(2019 novel coronavirus, 2019-nCoV)，同时在该患者的1份口咽拭子中分离到1株单纯疱疹病毒I型(herpes simplex virus type 1, HSV-1)。结论:COVID-19病例存在2019-nCoV和HSV-1合并感染的可能性。“,”Objective:To understand the etiology of a confirmed case of Coronavirus Disease 2019 (COVID-19).Methods:The pharyngeal swabs, serum and nasal swabs of a case of COVID-19 were inoculated into Vero-E6 cell tubes for virus isolation. The cytopathic effect (CPE) were observed daily. Collecting cell’s isolation when CPE was over 75%, after repeated freezing and thawing for 3 times, the supernatant was centrifugally taken, and the images of the virus were obtained by transmission electron microscopic observation, and the nucleic acid of the virus was extracted, second generation sequencing and sequence evolution analysis were used to identify and type the virus strains.Results:One strain of 2019 novel coronavirus (2019-nCoV) was successfully isolated from the nasal swab of this case of COVID-19, and one strain of herpes simplex virus type 1 (HSV-1) was also successfully isolated from the throat swab of the same case.Conclusions:COVID-19 cases have the possibility of co-infection with 2019-nCoV and HSV-1.