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目的 :建立大肠埃希菌定植的阴道感染小鼠模型。方法 :在阿莫西林溶液 (12 5mg/ml)冲洗小鼠阴道冲洗处理下 ,将致病性大肠埃希菌接种到小鼠阴道内。结果 :各组小鼠阴道冲洗液检出的平均对数值比较 ,经过阿莫西林溶液小鼠阴道冲洗处理后再接种大肠埃希菌的阿莫西林 +大肠埃希菌组 ,阴道内定植的大肠埃希菌数量 (8 18± 1 0 9)较经过生理盐水小鼠阴道冲洗处理后再接种大肠埃希菌组 (5 72± 0 6 8)明显增多 (P <0 0 5 ) ,而且小鼠阴道感染的症状明显 (P <0 0 5 )。结论 :通过抗生素处理后 ,再在小鼠阴道内接种大肠埃希菌 ,大肠埃希菌能够在小鼠阴道内得到定植 ,即建立起大肠埃希菌性阴道感染的小鼠模型。
Objective: To establish a vaginal infection mouse model of Escherichia coli colonization. Methods: Pathogenic Escherichia coli was inoculated into the vagina of mice under the vaginal washing of amoxicillin solution (125mg / ml). Results: The average logarithm of the vaginal washes in each group were compared. After the mice were treated by amoxicillin solution, the mice were inoculated with the amoxicillin + Escherichia coli group of Escherichia coli and the colon of the vagina The number of Escherichia coli (8 18 ± 1 0 9) was significantly increased (P <0 05) compared with that of E. coli group (5 72 ± 0 6 8) after inoculation of saline in mice, and the number of mice Symptoms of vaginal infections were significant (P <0 05). Conclusion: After antibiotic treatment, E. coli was inoculated intravaginally in mice and E. coli was colonized in the vagina of mice, that is, a mouse model of Escherichia coli vaginal infection was established.