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应用动态浊度法鲎试验定量测定硫酸庆大霉素注射液中的细菌内毒素含量。通过对样品中定量添加标准内毒素的干扰预试验 ,筛选出硫酸庆大霉素最佳的检测浓度 0 4mg·ml-1,进行正式干扰试验。样品中定量添加标准内毒素 ,5 0 0、 0 5 0、 0 0 5EU·ml-1,回收率为 5 0 %～ 2 0 0 % ,可用于有效的日常检查。
Determination of bacterial endotoxin in gentamicin sulfate injection by dynamic nephelometry. Through the pretreatment of quantitatively adding the standard endotoxin in the sample, the optimal detection concentration of gentamicin sulfate (0 4 mg · ml -1) was screened out for the formal interference test. Standard endotoxins were added to the samples at a level of 500, 050 and 0.005 eU · ml-1. The recoveries ranged from 50% to 200% and could be used for effective routine examination.