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目的:采用响应面法优化粗茎秦艽多糖的提取工艺。方法:基于单因素试验结果,选择对秦艽多糖提取影响较大的几个因素(溶剂倍数、提取温度、提取时间、提取次数)为自变量,使用BBD响应面优化设计对秦艽多糖的提取工艺条件进行优化,同时研究各自变量交互作用及其对秦艽多糖提取率的影响,模拟得到二次多项式回归方程的预测模型。结果:优化后,最佳提取工艺条件为:超声功率为200 W,超声时间为20 min,超声温度为25℃,溶剂倍数为14倍,提取时间为1.5 h,提取次数为2次,提取温度为80℃。在该提取条件下,秦艽多糖的实际提取率为4.36%与理论值4.55%相近。结论:实验值与预测值基本相符,该优化工艺稳定可靠。
OBJECTIVE: To optimize the extraction process of polysaccharide from Gentiana macrophylla by response surface methodology. Methods: Based on the single factor test results, several factors (solvent multiples, extraction temperature, extraction time and extraction times) which had a great influence on the extraction of polysaccharides from Gentiana macrophylla were selected as independent variables. The extraction conditions of polysaccharide from Gentiana macrophylla with BBD response surface were optimized. Optimize, and study the interaction of variables and their effects on extraction rate of polysaccharide from Gentiana macrophylla, and get the prediction model of quadratic polynomial regression equation. Results: The optimized extraction conditions were as follows: the ultrasonic power was 200 W, the ultrasonic time was 20 min, the ultrasonic temperature was 25 ℃, the solvent multiples were 14 times, the extraction time was 1.5 h, the extraction times were 2 times, the extraction temperature 80 ° C. Under this extraction condition, the actual extraction rate of polysaccharide from Gentiana macrophylla was 4.36%, which was close to the theoretical value of 4.55%. Conclusion: The experimental value is in good agreement with the predicted value, and the optimization process is stable and reliable.