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目的检测肝细胞肝癌、癌旁组织及肝硬化/慢性肝炎组织中中心体α-微管蛋白、P53蛋白及端粒酶的表达,探讨在肝细胞癌变过程中中心体异常与端粒酶的相关性。方法选取山东大学附属济南市中心医院病理科石蜡包埋组织肝细胞肝癌47例,相应的癌旁组织47例,肝硬化/慢性肝炎组织12例。采用免疫组织化学染色SP法检测中心体α-微管蛋白、P53蛋白及端粒酶逆转录酶hTERT蛋白;分析三者之间的相关性。结果中心体α-微管蛋白、P53蛋白在肝细胞肝癌中阳性表达率分别为59.57%、76.60%,明显高于癌旁组织23.40%、29.97%和肝硬化/慢性肝炎组织16.67%、33.33%,P均<0.05。肝癌组织中hTERT蛋白阳性表达率(68.09%)与癌旁组织(34.04%)、肝硬化/慢性肝炎组织(0%)比较差异均有统计学意义(P<0.05)。中心体α-微管蛋白、P53及hTERT三者间表达均呈正相关,提示三者在HCC癌变过程中可能存在协同作用。结论中心体异常参与了肝细胞癌变的过程,并有可能成为HCC新的诊断指标和治疗靶点。
Objective To detect the expression of α-tubulin, P53 protein and telomerase in hepatocellular carcinoma tissues, pericarcinoma tissues and cirrhosis / chronic hepatitis tissues, and to explore the correlation between the abnormal telomerase activity and centrosome in hepatocellular carcinoma Sex. Methods Forty-seven patients with hepatocellular carcinoma, 47 corresponding para-carcinoma tissues and 12 cirrhosis / chronic hepatitis tissues were selected from Department of Pathology, Jinan Central Hospital Affiliated to Shandong University. The centrosome α-tubulin, P53 protein and telomerase reverse transcriptase hTERT protein were detected by immunohistochemical SP method. The correlation between them was analyzed. Results The positive rates of α-tubulin and P53 protein in hepatocellular carcinoma were 59.57% and 76.60%, respectively, which were significantly higher than those in adjacent tissues (23.40%, 29.97%) and cirrhosis / chronic hepatitis (16.67% and 33.33% , P <0.05. The positive expression rate of hTERT protein in hepatocellular carcinoma tissue was significantly higher than that in adjacent tissues (34.04%) and cirrhosis / chronic hepatitis (0%) (68.09%) (P <0.05). There was a positive correlation between central α-tubulin, P53 and hTERT, suggesting that there may be synergistic effects among the three in carcinogenesis of HCC. Conclusion The abnormal centrosome is involved in the process of hepatocellular carcinogenesis and may become a new diagnostic and therapeutic target for HCC.