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目的:分析比较不同固相萃取纤维头(PA,PDMS,PDMS-DVB)对白花蛇舌草挥发性成分的吸附性,并与传统的水蒸气蒸馏联用GS-MS(SD-GS-MS)方法进行比较。方法:采用PA,PDMS-DVB,PDMS 3种不同类型的固相微萃取纤维头以及水蒸气蒸馏法提取,联用GS-MS进行挥发性成分测定。结果:其中PA检测出50个成分,PDMS-DVB检测出51个,PDMS检测出18个。水蒸气蒸馏法检测出41个成分,与SPME法对比结果显示,相似度最高的为PA,共有成分为21个,其次为PDMSDVB 15个,PDMS 6个;有16个成分未在SPME法中检出。结论:HS-SPME-GS-MS方法可简便、高效地检测白花蛇舌草挥发性成分,但不能完全替代传统的水蒸气蒸馏联用GS-MS方法。
OBJECTIVE: To compare and analyze the adsorption of volatile components of Hedyotis diffusa Willd. By different solid-phase extraction fiber heads (PA, PDMS, PDMS-DVB) and compare with the traditional steam distillation with GS-MS (SD- Method to compare. Methods: Three kinds of solid phase microextraction fiber heads (PA, PDMS-DVB and PDMS) were extracted by steam distillation, and the volatile components were determined by GS-MS. Results: PA was detected 50 components, PDMS-DVB detected 51, PDMS detected 18. 41 components were detected by steam distillation. Compared with SPME method, the highest similarity was PA with 21 common components, followed by 15 PDMSDVBs and 6 PDMSs, and 16 components were not detected by SPME method Out Conclusion: HS-SPME-GS-MS method can be used to detect the volatile components of Hedyotis diffusa easily and efficiently, but it can not completely replace the traditional steam distillation combined with GS-MS method.