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目的:探讨不同剂量的大豆异黄酮、维生素C和维生素E复合抗氧化剂协同作用对2型糖尿病患者糖负荷时血管内皮炎性细胞因子表达的影响。 方法:将120例符合条件的2型糖尿病患者按性别和体质量指数分层后随机分为4组(分别为糖尿病对照组、低剂量组、中剂量组、高剂量组),进行口服葡萄糖耐量试验(OGTT)时分别给予不同剂量的大豆异黄酮、维生素C和维生素E的混合干预物或安慰剂并与正常人(正常对照组)比较,观察各组在OGTF时0,1,2,4,6h血清中肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)的变化。 结果:4个2型糖尿病组空腹时TNF-α水平显著高于正常对照组(F=8.76,P<0.01)。OGTT中糖尿病对照组IL-6水平于餐后1h[(54.03±99.26)μg/L]达峰值,1,2 h均显著高于空腹[(54.65±104.70)μg/L](F=9.12,P<0.01;F=3.56,P<0.05);TNF-α水平于餐后2h[(67.26±95.05)μg/L]达峰值,2,4h均显著高于空腹(F=10.23,9.56,P<0.01)、1h(F=3.45,3.26,P<0.05)和6h(F=11.36,10.05,P<0.01)。低剂量组波动趋势同糖尿病对照组,IL-6水平餐后1h达高峰显著高于空腹(F=4.13,P<0.05);TNF-α水平餐后于餐后2h达峰值,2,4h均显著高于0 h(F=8.97,8.56,P<0.01)、1h(F=3.12,3.01,P<0.05)和6 h(F=9.01,8.87,P<0.01)。4h开始下降,至6 h时回落至空腹水平。中剂
Objective: To investigate the synergistic effects of different doses of soy isoflavones, vitamin C and vitamin E antioxidants on the expression of vascular endothelial proinflammatory cytokines in patients with type 2 diabetes during glycemic load. Methods: 120 eligible type 2 diabetic patients were randomly divided into 4 groups according to sex and body mass index (diabetic control group, low dose group, middle dose group, high dose group), oral glucose tolerance (OGTT) were given different doses of soy isoflavones, vitamin C and vitamin E mixed intervention or placebo and normal subjects (normal control group) were compared in each group observed OGTF 0,1,2,4 , 6 h serum TNF-α and IL-6. Results: The fasting serum TNF-αlevels in the four type 2 diabetic patients were significantly higher than those in the normal control group (F = 8.76, P <0.01). The level of IL-6 in OGTT diabetic control group reached peak at 1h [(54.03 ± 99.26) μg / L] and was significantly higher at 1,2 h than that at fasting [(54.65 ± 104.70) μg / L] (P <0.01; F = 3.56, P <0.05). The level of TNF-α peaked at 2 hours after meal (67.26 ± 95.05) μg / L, <0.01), 1h (F = 3.45,3.26, P <0.05) and 6h (F = 11.36,10.05, P <0.01). In the low-dose group, the trend of fluctuation was the same as that of the diabetic control group. The level of IL-6 peaked at 1h after meal was significantly higher than fasting (F = 4.13, P <0.05) (F = 8.97, 8.56, P <0.01), 1 h (F = 3.12, 3.01, P <0.05) and 6 h (F = 9.01, 8.87, P <0.01). 4h began to decline, to 6 h fell to fasting level. Medium dose