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对于建立T细胞克隆的两种方法中的一种方法而言,具有反复刺激培养中的抗原特异性和同种反应性T细胞的能力是很重要的基础。认识到有这种可能性已有4～5年时间了。在此期间,研究者探索着在体外进行再刺激,以进一步证实T细胞的特异性,特别是它们对抗原递呈细胞Ia组织相容性的要求。不久就发现,在培养末期少量存活的细胞(≤10%)中含有十分丰富的抗原特异性细胞。通过反复刺激,这些细胞的出现率可
The ability to repeatedly stimulate antigen-specific and alloreactive T cells in culture is a very important basis for one of the two methods of establishing T cell clones. It has been 4 to 5 years to recognize this possibility. In the meantime, the researchers explored in vitro restimulation to further confirm the specificity of T cells, in particular their requirement for histocompatibility with antigen presenting cells Ia. It was soon discovered that very few antigen-specific cells were present in the cells (<10%) that had survived in the late stage of culture. Through repeated stimulation, the appearance of these cells can be