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在人巨细胞病毒（HCMV）AD169株直接早期蛋白EcoRIJDNA片段内，自行设计二对引物，建立套式PCR检测HCMVDNA。经实验证明有高度的特异性与敏感性，对其它疙疹类病毒和正常人基因组DNA无交叉反应。一次性PCR检测HCMVAD169株基因组的最小检出量为100fg，而套式PCR可达10fg，经PStI酶切后，得到的片段与设计相符。对23例新生儿肝炎临床标本进行病毒分离，一次性PCR，套式PCR检测，结果表明套式PCR能进一步提高一次性PCR的特异性与敏感性，适用于新生儿肝炎HCMV感染的临测。
In the HCMV AD169 direct early EcoRIJDNA fragment, two pairs of primers were designed and nested PCR was used to detect HCMV DNA. The experiment proved a high degree of specificity and sensitivity, no other cross-reaction of the genus of other rash virus and normal human DNA. One-time PCR detection HCMVAD169 genome minimum detectable amount of 100fg, and nested PCR up to 10fg, after PStI digestion, the fragment was consistent with the design. The clinical isolates of 23 neonates with hepatitis were subjected to virus isolation, one-time PCR and nested PCR. The results showed that the nested PCR could further improve the specificity and sensitivity of one-time PCR and be suitable for the detection of neonatal hepatitis HCMV infection.