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为明确甘肃玉米大斑病菌对嘧菌酯的敏感基线和抗性水平,采用平皿法测定了采自甘肃4个典型生态区的57株玉米大斑病菌对嘧菌酯的敏感性。结果表明,甘肃玉米大斑病菌对嘧菌酯敏感性差异较大,EC50在0.03~5.21μg/m L之间,平均EC50为0.54μg/m L,其中有32个菌株EC50呈连续性正态分布,平均EC50为0.10μg/m L,即为甘肃玉米大斑病菌对嘧菌酯的敏感基线。基于此,发现12株抗性菌株,平均抗性水平为5.18,最高抗性水平为49.93,抗性频率为21.05%,其中南部湿润和陇东半湿润半干旱地区出现中、高抗菌株7株,占12.28%,陇东半湿润半干旱和中部干旱雨养地区出现低抗菌株5株,占8.77%,而河西干旱灌溉地区15株菌对嘧菌酯十分敏感,平均抗性水平为1.56,最高抗性水平为3.89,暂无抗药性。
In order to clarify the sensitive baseline and resistance of azoxystrobin against azoxystrobin in Gansu Province, the susceptibility of azoxystrobin to azoxystrobin in 57 isolates from four typical ecotopes in Gansu was determined by plate method. The results showed that the susceptibility of S. turcica to azoxystrobin was quite different with EC50 ranging from 0.03 to 5.21 μg / mL, with an average EC50 of 0.54 μg / mL. EC50 of 32 strains showed continuous normality Distribution, the average EC50 of 0.10μg / m L, is the Gansu virulent base of azoxystrobin susceptible base. Based on this, 12 resistant strains were found, with an average resistance level of 5.18, a highest resistance level of 49.93 and a frequency of 21.05%. Seven middle and high resistant strains were found in southern humid and semi-humid semiarid regions of eastern Gansu. , Accounting for 12.28%. There were 5 low-resistant strains in semi-humid semi-arid and middle arid rainy areas of eastern Gansu Province, accounting for 8.77%, while 15 isolates in arid irrigated area of Hexi were very sensitive to azoxystrobin and the average resistance level was 1.56, The highest level of resistance was 3.89, no drug resistance.