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目的观察研究C57BL/6J小鼠肾缺血再灌注期间颈部迷走神经放电变化,并介绍其迷走神经放电采集方法与技巧。方法雄性C57BL/6J小鼠8只,8~10周龄,体质量22~26 g,沿颈正中线切开皮肤,逐层分离至颈迷走神经,用铂金电极头引导神经放电并记录其放电频率;继而进行腹部手术,通过夹闭双侧肾门缺血25 min后再恢复血流30 min,该过程持续记录其迷走神经放电频率变化。结果与基础放电频率相比,肾缺血期间迷走神经放电频率由(520±52)Hz上升至(632±59)Hz(n=8,P<0.05),再灌注后15 min内放电频率进一步升高至(672±58)Hz(n=8,P<0.05),之后逐步下降至(544±51)Hz(n=8,P<0.05)。结论肾缺血再灌注期间小鼠颈部迷走神经放电频率较基础放电频率明显上升,其原因可能涉及缺血再灌注时自身的神经保护性机制。
Objective To observe the changes of cervical vagal discharge during C57BL / 6J renal ischemia / reperfusion in rabbits and to introduce the methods and skills of vagal nerve discharge acquisition. Methods Eight male C57BL / 6J mice aged 8-10 weeks with a body weight of 22-26 g were divided into cervical vagotomy along the median line of the neck and neuro discharges were guided by platinum electrodes and the discharge frequency was recorded ; Followed by abdominal surgery, by clamping bilateral renal ischemia 25 minutes before resuming blood flow 30 min, the process of continuous recording of changes in vagal discharge frequency. Results Compared with the basal discharge frequency, the frequency of vagal discharge increased from (520 ± 52) Hz to (632 ± 59) Hz (n = 8, P <0.05) during renal ischemia and further increased within 15 min after reperfusion (672 ± 58) Hz (n = 8, P <0.05), then gradually decreased to (544 ± 51) Hz (n = 8, P <0.05). Conclusion The frequency of cervical vagal nerve discharge during renal ischemia-reperfusion in rats is significantly higher than that of basal discharge, which may be related to the neuroprotective mechanism during ischemia-reperfusion.