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目的:探讨CD4+CD25+调节性T细胞(即CD4+CD25+Treg细胞)在卵巢早衰发病机制中的作用。方法:流式细胞仪定量检测卵巢早衰(premature ovarian failure,POF)患者、卵巢储备功能下降(diminished ovarian reserve,DOR)患者及健康对照组外周血CD4+T、CD8+T细胞及CD4+CD25+Treg细胞数量,应用3H-thymidine掺入法测定POF患者及对照组外周血CD4+CD25+Treg细胞对效应性T细胞的增殖抑制功能。结果:与对照组相比,POF患者及DOR患者CD4+CD25+Treg细胞比例降低(P<0.01)、POF患者CD4+T/CD8+T细胞比值增高(P<0.05),DOR患者CD4+T/CD8+T细胞比值无明显变化(P>0.05);POF患者免疫抑制功能无明显降低(P>0.05)。结论:CD4+CD25+Treg细胞比例降低与T细胞亚群失衡可能是POF的发病机制。
Objective: To investigate the role of CD4 + CD25 + regulatory T cells (CD4 + CD25 + Treg cells) in the pathogenesis of premature ovarian failure. Methods: Flow cytometry was used to detect the levels of CD4 + T, CD8 + T cells and CD4 + CD25 + T cells in peripheral blood of patients with premature ovarian failure (POF), diminished ovarian reserve (DOR) and healthy controls. Treg cell number, 3H-thymidine incorporation method was used to determine the inhibitory effect of CD4 + CD25 + Treg cells on the proliferation of effector T cells in POF patients and control group. Results: Compared with the control group, the proportion of CD4 + CD25 + Treg cells in POF patients and DOR patients was decreased (P <0.01), the ratio of CD4 + T / CD8 + T cells in POF patients was increased / CD8 + T cell ratio (P> 0.05). The immunosuppressive function of POF patients was not significantly reduced (P> 0.05). Conclusion: The decreased proportion of CD4 + CD25 + Treg cells and the imbalance of T cell subsets may be the pathogenesis of POF.