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目的 检测尿激酶型纤溶酶原激活物 (uPA)及其受体uPAR在类风湿关节炎 (RA)患者滑液和血浆中的含量 ,并结合临床和实验室资料分析其实际意义。方法 运用酶联免疫吸附法(ELISA)双抗体夹心法分别测定 46例RA、8例骨关节炎 (OA)和 12名健康对照个体的血浆以及 14例RA患者滑液中的uPA和uPAR含量 ,同时将类风湿因子阳性 (RF+ )和阴性 (RF-)组之间uPA和uPAR含量进行比较 ,并采用直线相关与回归方法分析血浆和滑膜中uPA、uPAR的含量与临床上评价RA病情活动指标之间的相互关系。结果 RA患者滑液中uPA、uPAR的含量均显著高于其自身血浆 (P <0 0 0 1,P <0 0 1) ;RA血浆中uPA、uPAR的含量又明显高于OA组 (P <0 0 5 ,P <0 0 0 0 1)和正常对照组 (P <0 0 0 0 1,P <0 0 0 1) ;RF+ 和RF-组之间uPA、uPAR在滑液和血浆中的含量差异均无显著性 (P >0 0 5 )。RA滑液和血浆中uPA、uPAR的含量与反映RA病情活动的指标C反应蛋白 (CRP)和类风湿因子 (RF)之间均呈正相关 ;RA滑液中uPA、uPAR的含量还与关节肿胀个数呈正相关。结论 RA患者滑液和血浆中uPA和uPAR的含量是反映RA病情活动的有用指标 ,提示uPA和uPAR基因可能通过降解细胞外基质的蛋白裂解作用参与RA的免疫作用机制并发挥重要作用
Objective To detect the content of urokinase-type plasminogen activator (uPA) and its receptor uPAR in synovial fluid and plasma of patients with rheumatoid arthritis (RA) and to analyze its clinical significance with clinical and laboratory data. Methods The levels of uPA and uPAR in synovial fluid of 46 patients with RA, 8 patients with osteoarthritis (OA) and 12 healthy controls and 14 RA patients were determined by enzyme-linked immunosorbent assay (ELISA) At the same time, the levels of uPA and uPAR between the RF + and RF- groups were compared, and the contents of uPA and uPAR in plasma and synovium were analyzed by linear correlation and regression, The relationship between indicators. Results The levels of uPA and uPAR in synovial fluid of RA patients were significantly higher than those of their own plasma (P <0.01 or P <0.01). The levels of uPA and uPAR in RA patients were significantly higher than those in OA patients (P < 0 0 0, P 0 01 0) and normal control group (P 0 01 0 1, P 0 01 0); uPA and uPAR between RF + and RF-group in synovial fluid and plasma There was no significant difference (P> 0.05). RA synovial fluid and plasma levels of uPA and uPAR were positively correlated with CRP and rheumatoid factor (RF), which reflect RA’s disease activity. The content of uPA and uPAR in RA synovial fluid was also correlated with joint swelling The number of positive correlation. Conclusion The levels of uPA and uPAR in synovial fluid and plasma of patients with RA are useful indicators of RA activity, suggesting that uPA and uPAR may play important roles in the immune mechanism of RA by degrading the extracellular matrix