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目的检测类风湿关节炎(RA)患者外周血单个核细胞(PBMC)中miR-146a的表达情况,并探讨其与RA病情活动性及临床表现的相关性。方法采用实时荧光定量聚合酶链反应方法,对82例RA患者(活动期47例,非活动期35例)及40例健康对照者PBMC中的miR-146a的表达水平进行检测及分析。结果 RA患者PBMC中miR-146a的表达水平(1.70±0.39)显著高于健康对照者(0.63±0.04),差异有统计学意义(P<0.05);活动期RA患者PBMC中miR-146a的表达水平(1.91±0.05)显著高于非活动期RA患者(1.42±0.04),差异有统计学意义(P<0.05);非活动期RA患者PBMCs中miR-146a的表达水平与正常对照组比较,差异无统计学意义(P>0.05)。有关节外表现的RA患者PBMC中miR-146a的表达水平(2.13±0.12)显著高于无关节外表现的RA患者(1.59±0.04),差异有统计学意义(P<0.05)。miR-146a的表达水平与RA患者的DAS28评分呈正相关性(r=0.676,差异有统计学意义,P<0.05)、与血沉(ESR)呈正相关性(r=0.660,差异有统计学意义,P<0.05)、与C-反应蛋白(CRP)水平呈正相关性(r=0.518,差异有统计学意义,P<0.05),但与病程、类风湿因子(RF)及抗环瓜氨酸肽抗体(抗CCP抗体)无明显相关性(P>0.05)。结论 miR-146a在RA患者PBMC中的表达显著增高,并与RA病情活动度及特定临床表现相关,提示miR-146a可能参与了RA的发病,检测其在外周血单个核细胞的表达可能有助于RA的诊断及病情活动性的判断。
Objective To detect the expression of miR-146a in peripheral blood mononuclear cells (PBMCs) from patients with rheumatoid arthritis (RA) and to explore the relationship between the expression of miR-146a and the activity and clinical manifestations of RA. Methods The expression of miR-146a in PBMC from 82 patients with RA (47 active, 35 inactive) and 40 healthy controls were detected by real-time fluorescence quantitative polymerase chain reaction. Results The expression level of miR-146a in PBMC of RA patients (1.70 ± 0.39) was significantly higher than that of healthy controls (0.63 ± 0.04), the difference was statistically significant (P <0.05); The expression of miR-146a (1.91 ± 0.05) was significantly higher than that of inactive RA patients (1.42 ± 0.04), the difference was statistically significant (P <0.05). The expression of miR-146a in non-active RA patients PBMCs compared with the normal control group, The difference was not statistically significant (P> 0.05). The expression level of miR-146a in peripheral blood mononuclear cells (2.13 ± 0.12) in RA patients with extra-articular manifestations was significantly higher than that in RA patients without extra-articular manifestations (1.59 ± 0.04), the difference was statistically significant (P <0.05). The expression of miR-146a was positively correlated with DAS28 score in RA patients (r = 0.676, P <0.05), and was positively correlated with ESR (r = 0.660, the difference was statistically significant, (R = 0.518, the difference was statistically significant, P <0.05), but with the course of disease, rheumatoid factor (RF) and anti-cyclic citrullinated peptide Antibody (anti-CCP antibody) no significant correlation (P> 0.05). Conclusion The expression of miR-146a in PBMC of patients with RA is significantly increased, which is correlated with the activity of RA and the specific clinical manifestations. It suggests that miR-146a may be involved in the pathogenesis of RA and its expression in peripheral blood mononuclear cells may be helpful Diagnosis of RA and determination of disease activity.