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目的:分析胃癌患者Th1和Th2两类细胞因子的基因表达与转录因子T-bet和GATA3表达的相关性,从细胞因子与转录因子角度考证胃癌患者Th1/Th2细胞分化趋势,探讨p53与T-bet的相关性,了解T-bet与肿瘤转移之间的关系。方法:利用荧光定量PCR技术检测55例胃癌患者及45例正常人外周血单个核细胞(PBMC)中T-bet、GATA3转录因子和IFN-γ、IL-4等细胞因子的水平,应用免疫组化法检测胃癌组织中p53的表达状况。结果:55例胃癌患者T-bet、GATA3、IFN-γ、IL-4的表达率依次为51%(28/55)、78%(43/55)、27%(15/55)、69%(38/55)。定量PCR结果显示,病人组的T-bet和IFN-γ明显低于正常对照组(P<0.01),而GATA3和IL-4则明显高于正常对照组(P<0.01)。T-bet与IFN-γ在病人体内的mRNA表达存在正相关性(P<0.01),正常人则没有。GATA3与IL-4的表达在两组中均呈明显正相关(P<0.01和P<0.05),且在p53阳性的患者中,T-bet的表达率较低,而GATA3的表达率较高。结论:胃癌患者有明显Th2漂移现象,且p53的表达与T-bet的表达呈负相关。
OBJECTIVE: To analyze the correlation between the expression of Th1 and Th2 cytokines and the expressions of T-bet and GATA3 in gastric cancer patients. To investigate the differentiation of Th1 / Th2 cells from the perspective of cytokines and transcription factors, bet, to understand the relationship between T-bet and tumor metastasis. Methods: The levels of T-bet, GATA3, IFN-γ, IL-4 and other cytokines in peripheral blood mononuclear cells (PBMCs) of 55 gastric cancer patients and 45 healthy controls were detected by real-time fluorescence quantitative PCR Method to detect the expression of p53 in gastric cancer. Results: The expression rates of T-bet, GATA3, IFN-γ and IL-4 in 55 gastric cancer patients were 51% (28/55), 78% (43/55), 27% (38/55). Quantitative PCR results showed that T-bet and IFN-|Ã in patients were significantly lower than those in normal control group (P <0.01), but GATA3 and IL-4 were significantly higher than those in normal control group (P <0.01). T-bet and IFN-γ mRNA expression in the patient there is a positive correlation (P <0.01), normal people did not. The expression of GATA3 and IL-4 were positively correlated (P <0.01 and P <0.05) in both groups, and the expression of T-bet was lower in patients with p53-positive, while the expression of GATA3 was higher . Conclusions: There is a significant Th2 shift in gastric cancer patients, and the expression of p53 is negatively correlated with the expression of T-bet.